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TRIzol® Reagents are ready-to-use, monophasic solutions of phenol and guanidine isothiocyanate suitable for isolating total RNA, DNA, and proteins (1-6). Isolation procedures are based upon improvements to the single-step RNA isolation method Three nucleic acid extraction processes were evaluated: Trizol LS® (Life Technologies, Saint Aubin, France), the QIAmp viral RNA mini kit (Qiagen, Courtaboeuf, France), and the High Pure viral nucleic acid kit (Roche Diagnostics, Meylan, France) according to the manufacturer's protocols.